| Species |
Human |
| Protein Construction |
Expressed with an N-terminal Met and several site mutations
Human Heat Stable FGF-basic (Ala135-Ser288)
Accession # P09038 |
|
| Purity |
≥ 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 0.25 ng/ml as determined by a dose-response proliferation assay using 3T3 cells. Based on the ED50, the calculated specific activity is approximately > 4.0 × 106 units/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay. |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
17.1 kDa |
| Apparent Molecular Weight |
~17.1 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in PBS up to 100 μg/ml |
| Storage & Stability |
Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C. |
The ED50 as determined by a cell proliferation assay using 3T3 cells is 0.04 ng/ml.
Lane 1: 2 µg of Heat Stable FGF-basic, Human, reducing (R)
The purity is greater than 95% as analyzed by SDS-PAGE
| Target Background |
Heat Stable FGF-basic, Human is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomyocytes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury. |
| Synonyms |
Heat Stable FGF-2 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
