| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | ... The immunocomplexes were isolated by the addition of 100 μl of protein G Magbeads (Genscript, Piscataway, NJ), followed by incubation for 3–4 h at 4 °C. The immunoprecipitates were then washed four times with PBS, resuspended in Laemmli sample buffer, and boiled for 5 ... | Get A Quote |
Akt is a serine/threonine kinase, which is dramatically reduced in the prefrontal cortex (PFC) of patients with schizophrenia, and a deficiency in Akt1 results in PFC function abnormalities. Although the importance of Akt in dopamine (DA) transmission is well established, how impaired Akt signaling affects the DA modulation of synaptic transmission in the PFC has not been characterized. Here we show that Akt inhibitors significantly decreased receptor sensitivity to DA by shifting DA modulation of GABAA receptor-mediated inhibitory postsynaptic currents (IPSCs) in prefrontal cortical neurons. Akt inhibition caused a significant decrease in synaptic dopamine D2 receptor (D2R) levels with high-dose DA exposure. I... More
