Newcastle disease virus (NDV) and Salmonella Pullorum (S. Pullorum) have great damage to the poultry industry, but no perfect vaccine can protect them effectively until now. In this study, a recombinant-attenuated S. Pullorum strain secreting Newcastle disease virus hemagglutinin-neuraminidase (HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed as a new bivalent vaccine candidate against Newcastle disease and S. Pullorum disease (PD) by the method of suicide plasmid pREasd-mediated bacteria homologous recombination. The effect of this vaccine candidate was compared with NDV LaSota strain and C79-13ΔcrpΔasd (pYA) strain. The serum HI antibody titers, serum IgG antibodies, the intestinal sIgA and stimulation index in lymphocyte proliferation were significantly higher (P<0.01) induced and increased in chickens inoculated with C79-13ΔcrpΔasd (pYA-HN) strains compared with that of the C79-13ΔcrpΔasd (pYA) strain and were not significantly increased compared with that in the chicken immunized with LaSota live vaccine (P>0.05). And it could provide 60% and 80% protective efficacy against NDV virulent strain F48E9 and S. Pullorum virulent strain C79-13. In summary, the results indicate that recombinant-attenuated S. Pullorum (SP) strain secreting NDV HN protein was constructed, and the generation of C79-13ΔcrpΔasd (pYA-HN) strain provides a good foundation for the development of an effective living-vector double vaccine against NDV and S. Pullorum.