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A spectroscopic sensing platform for MARCKS protein monolayers.

J Colloid Interface Sci.. 2017-12; 
Klug J, Torresan MF, Lurgo F, Borioli G, Lacconi GI.
Products/Services Used Details Operation
PCR Cloning and Subcloning ... NaCl (MERCK). The MARCKS protein was purified by affinity chromatography using pETMARCKS, constructed by cloning the human MARCKS cDNA (996 bp) in the pET15-B vector (GenScript, USA Inc) [33]. Protein stocks ... Get A Quote

摘要

We developed a highly sensitive silicon platform, suitable to assess the molecular organization of protein samples. Prototype platforms were obtained using different electrochemical protocols for the electrodeposition of Ag-nanoparticles onto the hydrogenated silicon surface. A platform with high Surface Enhanced Raman Scattering efficiency was selected based on the surface coverage and the number density of particles size distribution. The performance of the platform was determined by studying the interaction of Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) protein with the substrate according to its molecular organization. The chemical and structural characteristics of MARCKS molecules were examined ... More

关键词

Intrinsically unstructured proteins; Langmuir-Blodgett monolayers; MARCKS; SERS active platforms; Silver nanoparticles electrodeposition
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