Chagas cardiomyopathy， caused by Trypanosoma cruzi infection， continues to be a neglected illness， and has a major impact on global health. The parasite undergoes several stages of morphological and biochemical changes during its life cycle， and utilizes an elaborated antioxidant network to overcome the oxidants barrier and establish infection in vector and mammalian hosts. Trypanothione synthetase (TryS) catalyzes the biosynthesis of glutathione-spermidine adduct trypanothione (T(SH)) that is the principal intracellular thiol-redox metabolite in trypanosomatids.

Chagas disease，Trypanosoma cruzi，anti-parasite drugs，paullones，small molecule inhibitors，trypanothione synthe