Products/Services Used | Details | Operation |
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Codon Optimization> | … Zebrafish codon-usage optimization of CreER T2. Zebrafish codon-usage optimization was carried out by GenScript USA Inc., using the OptimumGene™ algorithm … S1) generated by GenScript USA Inc., along with a polyA (pA) sequence … | Get A Quote |
New genome-editing approaches, such as the CRISPR/Cas system, have opened up great opportunities to insert or delete genes at targeted loci and have revolutionized genetics in model organisms like the zebrafish. The Cre-loxp recombination system is widely used to activate or inactivate genes with high spatial and temporal specificity. Using a CRISPR/Cas9-mediated knock-in strategy, we inserted a zebrafish codon-optimized CreER transgene at the otx2 gene locus to generate a conditional Cre-driver line. We chose otx2 as it is a patterning gene of the anterior neural plate that is expressed during early development. By knocking in CreER upstream of the endogenous ATG of otx2, we utilized this gene's nati... More