| Products/Services Used | Details | Operation |
|---|---|---|
| Monoclonal Antibody Services | … Bio-Rad). The membrane was incubated for 1 h at room temperature with 5% dry skimmed milk in PBS and then, for an extra hour in a renewed solution containing mouse anti-GST monoclonal antibody (GenScript, USA). After … | Get A Quote |
Influenza is a highly contagious respiratory tract disease and is considered a serious community health problem. Influenza viruses possess multiple conserved epitopes which are used for designing universal vaccines. To this aim， the gene coding for N-terminal part of M2e (SLLTEVET) and HA2 (GLFGAIAGF)， was synthesized， linked by a (Gly4Ser) peptide linker， and cloned into pGS-21a vector. Afterwards， the construct was transferred into E. coli BL21 (DE3) cells to produce the designed antigenic protein called M2e-HA2. Immunization of mice with these peptides significantly induced humoral immune responses against the influenza virus. Three weeks after the last booster， mice were inoculated intranasally ... More
