Egg proteins are a good source of bioactive peptides. Previous work from our research group has demonstrated the potential of egg white hydrolysate (EWH) for enhancing adipogenic differentiation and insulin signalling in 3T3-F442A pre-adipocytes. EWH was prepared by a combination of thermolysin and pepsin. Here， in this study， we aimed to identify the responsible peptide(s) in EWH. EWH was fractionated stepwise by ultrafiltration， C18 Sep-Pack cartridge， cation-exchange chromatography， and reverse-phase chromatography. The two most active fractions were analyzed by LC-MS/MS and 42 peptides were identified. Eleven peptides were synthesized and WEKAFKDED， QAMPFRVTEQE， ERYPIL， and VFKGL from ovalbumin were validated with peroxisome proliferator-associated receptor gamma stimulatory activity in adipocytes. For the first time， adipogenic differentiating peptides were characterized from egg white proteins. This data is pivotal for future structure-function studies of adipogenic peptides.