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A nebulin super-repeat panel reveals stronger actin binding towards the ends of the super-repeat region.

Muscle Nerve. 2018-09;

LaitilaJenni，LehtonenJohanna，LehtokariVilma-Lotta，SagathLydia，Wallgren-PetterssonCarina，GrönholmMikaela，PelinKata

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PCR and Cloning	… different nebulin SRs were constructed by RT-PCR and T-vector cloning (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), four SRs were constructed for our previous study, 17 and five were synthetised by GenScript (Piscataway, NJ, USA). The SR fragments …	Get A Quote

摘要

Nebulin is a giant actin-binding protein in the thin filament of the skeletal muscle sarcomere. Studies of nebulin interactions are limited by the size， complexity and poor solubility of the protein. We divided the nebulin super-repeat region into a super-repeat panel， and studied nebulin/actin interactions.