Products/Services Used | Details | Operation |
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Peptide Synthesis> | … Peptides were synthesized by F-moc solid-phase peptide synthesis from GenScript Biotech (Nanjing, China) with ≥ 95% purity. The sequences of the synthesized peptides were shown in Table 1. Table 1 Sequences of CVP1, TAT peptide and analogs used in the study. No … | Get A Quote |
Cell-penetrating peptide (CPP) is a promising cargo for delivering bioactive molecules. In this study, the N terminus of VP1 from chicken anemia virus, designated as CVP1, was found to carry enriched arginine residues with α-helix. By confocal imaging, flow cytometry and MTT assay, we identified CVP1 as a novel, safe and efficient CPP. CVP1-FITC peptide could entry different types of cells tested with dose dependence, but without cytotoxic effects. Compared with TAT-FITC peptide, the CVP1-FITC peptide showed much higher cell-penetrating activity. Moreover, CVP1 could successfully deliver β-glycosidase, poly (I:C) and plasmid into HCT116 cells. Inhibitors and temperature sensitivity analysi... More