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Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice.

Rice (N Y). 2018-04;

ChuThi Thu Huyen，HoangThi Giang，TrinhDuy Chi，BureauCharlotte，MeynardDonaldo，VernetAurore，IngouffMathieu，DoNang Vinh，PérinChristophe，GuiderdoniEmmanuel，GantetPascal，MaurelChristophe，LuuDoan-T

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Proteins, Expression, Isolation and Analysis	… A molecular construct consisting of the maize ubiquitin-1 promoter controlling the expression of a fluorescent plasma-membrane-localized fusion protein (ECFP-LTI6a) (Cutler et al. 2000) was obtained by synthesis (Genscript) and cloned into the plasmid pUC57 …	Get A Quote

摘要

Cell biology approach using membrane protein markers tagged with fluorescent proteins highlights the dynamic behaviour of plant cell membranes， not only in the standard but also in changing environmental conditions. In the past， this strategy has been extensively developed in plant models such as Arabidopsis.