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Novel one-pot ATP regeneration system based on three-enzyme cascade for industrial CTP production

Biotechnol. Lett.. 2017-12; 
WangJunzhi, ZhengCheng, ZhangTianyi, LiuYingmiao, ChengZhuopei, LiuDong, YingHanjie, NiuHuan
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Codon Optimization … EcPPK from E. coli; MtPPK from M. tuberculosis and TthPPK from T. thermophilus were codon-optimized by GenScript … EcNDK from E. coli, MtNDK from M. tuberculosis and TthPPK from T. thermophilus were codon-optimized by GenScript … Get A Quote

摘要

To develop a new one-pot polyphosphate kinase (PPK) system with low cost and high efficiency for ATP regeneration in industrial CTP production.

关键词

ATP regeneration system，CTP production，Fusion expression，Molecular chaperone，Polyphosphate ki
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