3-Hydroxypropanoic acid (3-HP) is an important biomass-derivable platform chemical that can be converted into a number of industrially relevant compounds. There have been several attempts to produce 3-HP from renewable sources in cell factories， focusing mainly on ， and . Despite the significant progress made in this field， commercially exploitable large-scale production of 3-HP in microbial strains has still not been achieved. In this study， we investigated the potential of as a microbial platform for bioconversion of glycerol into 3-HP. Our recombinant strains overexpress the two-step heterologous pathway containing glycerol dehydratase and aldehyde dehydrogenase from . Genetic engineering， driven by optimization， and optimization of cultivation conditions resulted in a 3-HP titer of 10 g/L， in a standard batch cultivation. Our findings provide the first report of successful introduction of the biosynthetic pathway for conversion of glycerol into 3-HP in . With this relatively high titer in batch， and the robustness of in high density fermentation conditions， we expect that our production strains may constitute a solid basis for commercial production of 3-HP.