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Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells.

Int J Ophthalmol. 2017-01; 
LiuGuo-Dan, ZhouShi-Feng, DingXu-Chen, FangChun-Lai, MiShu-Yong, GaoXiang-Chun, Han
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Catalog Antibody … Fetal Bovine Serum (FBS), Dulbecco's Modified Eagle Medium (DMEM), ampicillin, chloramphenicol, and Alexa488-conjugated secondary antibody were purchased from Invitrogen (Carlsbad, CA, USA). TAT and NLS sequences were synthesized at GenScript (Nanjing, China) … Get A Quote

摘要

To develop a new method to produce recombinant reprogramming proteins, cMyc, Klf4, Oct4, and Sox2, in soluble format with low cost for the generation of induced pluripotent stem cells (iPSCs).

关键词

induced pluripotent stem cells,protein transduction,reprogramming proteins,soluble expres
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