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| Gene Synthesis | … 2. MATERIALS AND METHODS 2.1. Materials E. coli strains TOP 10 and BL21 (DE3) were purchased from Invitrogen and Novagen, respectively. The cDNA of fp15 and all the DNA primers (Table 1) were synthesized by Genscript Corporation. Restriction endonucleases were … | Get A Quote |
Since cell adhesion is important for cell processes such as migration and proliferation， it is a crucial consideration in biomaterial design and development. Based on the fusion of mussel adhesive protein fp151 with laminin-1-originated functional peptides we designed fusion proteins (fLA4， fLG6 and fAG73) and explored their cell adhesion properties. In our study， cell adhesion analysis showed that protein fLG6 and fLA4 had a significantly higher cell adhesion property for A549 than fp151. Moreover， protein fAG73 also displayed a strong adhesion capacity for Hela cells. In conclusion， the incorporation of functional peptides with integrin and heparin/heparan sulphate binding capacity into mussel adhes... More
