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Codon Optimization> | … Standard DNA digestions and ligations were carried out according to the manufacturer's instructions. Phage tail genes were codon optimized (Table S1) and synthesized by GenScript. The plasmids that were used in this study are listed in Table S3 … | Get A Quote |
A major limitation in using bacteriophage-based applications is their narrow host range. Approaches for extending the host range have focused primarily on lytic phages in hosts supporting their propagation rather than approaches for extending the ability of DNA transduction into phage-restrictive hosts. To extend the host range of T7 phage for DNA transduction, we have designed hybrid particles displaying various phage tail/tail fiber proteins. These modular particles were programmed to package and transduce DNA into hosts that restrict T7 phage propagation. We have also developed an innovative generalizable platform that considerably enhances DNA transfer into new hosts by artificially selecting tails that e... More