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Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2.

BMC Vet. Res.. 2019-01; 
ZhuangLinlin, JiYongxin, TianPeilong, WangKaixuan, KouChengkun, GuNing, Zha
Products/Services Used Details Operation
PCR Cloning and Subcloning … Finally, the PCR products were purified with Magbeads PCR Purification Kit (Nanoeast, Nanjing, China) for genomic sequencing (GenScript, Nanjing, China) … Positive clones were identified by sequencing (GenScript, Nanjing, China) … Get A Quote

摘要

Canine parvovirus 2 (CPV-2) is one of the most common etiological agents that cause severe gastroenteritis in puppies. Early accurate diagnosis is important for infected dogs. In recent years, magnetic separation has become an efficient and useful tool for bioassays. In this study, polymerase chain reaction (PCR) combined with fluorescent lateral flow immunoassay (LFIA) based on magnetic purification assay was developed for the quantitative detection of CPV-2.

关键词

Canine parvovirus,Fluorescent lateral flow immunoassay,Magnetic purification,Polymerase chain reac
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