| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Three polymorphic region of SDS2 (indica allele) were selected to raise antibodies in rabbit. Two of them are located at extracellular domain and another is at intracellular domain. The peptides antigen were chemically synthesized and coupled with a single cysteine at N-termini. The sequences of peptides are: QKEGKEKSHPHHS, IPNARLPDNGKKLT and SAEMDAHDGSFSQN. The synthesis peptides and immunization of animals were carried by GenScript (Beijing, China). | Get A Quote |
Programmed cell death (PCD) plays critical roles in plant immunity but must be regulated to prevent excessive damage. The E3 ubiquitin ligase SPL11 negatively regulates PCD and immunity in plants. We show that SPL11 cell-death suppressor 2 (SDS2)， an S-domain receptor-like kinase， positively regulates PCD and immunity in rice by engaging and regulating SPL11 and related kinases controlling defense responses. An sds2 mutant shows reduced immune responses and enhanced susceptibility to the blast fungus Magnaporthe oryzae. Conversely， SDS2 over-expression induces constitutive PCD accompanied by elevated immune responses and enhanced resistance to M. oryzae. SDS2 interacts with and phosphorylates SPL11， wh... More
