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A novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses.

Sci Rep. 2018; 
SaarinenNiila V V,LaihoJutta E,RichardsonSarah J,ZeisslerMarie,StoneVirginia M,MarjomäkiVarpu,KantoluotoTino,HorwitzMarc S,Sioofy-KhojineAmirbabak,HonkimaaAnni,HankaniemiMinna M,Flodström-TullbergMalin,HyötyHeikki,HytönenVesa P,LaitinenOl
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Catalog Antibody Te protocol was approved by the Animal Care Committee of the University of British Columbia (certifcate number A13-0116). Te parafn embedded human tissue samples used in this study were from the UK collection30 or from the PanFin network26. Handling of rats was performed by Genscript. Get A Quote

摘要

Enteroviruses (EVs) are common RNA viruses that cause diseases ranging from rash to paralytic poliomyelitis. For example, EV-A and EV-C viruses cause hand-foot and mouth disease and EV-B viruses cause encephalitis and myocarditis, which can result in severe morbidity and mortality. While new vaccines and treatments for EVs are under development, methods for studying and diagnosing EV infections are still limited and therefore new diagnostic tools are required. Our aim was to produce and characterize new antibodies that work in multiple applications and detect EVs in tissues and in vitro. Rats were immunized with Coxsackievirus B1 capsid protein VP1 and hybridomas were produced. Hybridoma clones were selec... More

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