| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | AfFtn gene was selected based on the sequence in GenBank AF_RS04235. The gene with mutations for C-terminus truncation, as well as altered charges was synthesized from GenScript.The genes for GFPuv (based on the wild-type GFP sequence P42212 with three mutations F99S, M153T, and V163A), HRPc (P00433), and rLuc (P27652) were synthesized from Genscript, digested using SpeI and EagI restriction enzymes, and the resulting fragments ligated with pBAD/HisB using Expresslink T4 DNA ligase. | Get A Quote |
The expression and stabilization of recombinant proteins is fundamental to basic and applied biology. Here we have engineered a thermostable protein nanoparticle (tES) to improve both expression and stabilization of recombinant proteins using this technology. tES provides steric accommodation and charge complementation to green fluorescent protein (GFPuv)， horseradish peroxidase (HRPc)， and Renilla luciferase (rLuc)， improving the yields of functional in vitro folding by ~100-fold. Encapsulated enzymes retain the ability to metabolize small-molecule substrates， presumably via four 4.5-nm pores present in the tES shell. GFPuv exhibits no spectral shifts in fluorescence compared to a nonencapsulated contr... More
