Products/Services Used | Details | Operation |
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Peptide Synthesis> | All peptides were purchased from GenScript, Piscataway, NJ. The chemically synthesized peptides were purified, lyophilized, and analyzed by MS and HPLC to ensure ≥70% purity by the company. | Get A Quote |
Synthetic control strategies using signaling peptides to regulate and coordinate cellular behaviors in multicellular organisms and synthetic consortia remain largely underdeveloped because of the complexities necessitated by heterologous peptide expression. Using recombinant proteins that exploit split intein-mediated reactions, we presented here a new strategy for reconstituting functional signaling peptides capable of eliciting desired cellular responses in S. cerevisiae. These designs can potentially be tailored to any signaling peptides to be reconstituted, as the split inteins are promiscuous and both the peptides and the reactions are amenable to changes by directed evolution and other protein engine... More