| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | The lysates were resolved via 6% SDSPAGE (for the dmTOR protein) or 10% SDS-PAGE (for internal control GAPDH protein), transferred to a PVDF membrane (Millipore, Bedford, MA, USA) and probed with anti-dmTOR or anti-GAPDH antibodies (Santa Cruz Biotechnology, CA, USA). Anti-dmTOR antibodies were polyclonal antibodies that were custom-made by GenScript USA Inc. (Nanjing, China). The epitope was predicted using the GenScript OptimumAntigen design tool, and the peptide antigen was then synthesized. | Get A Quote |
The major environmental determinants of honeybee caste development come from larval nutrients: royal jelly stimulates the differentiation of larvae into queens， whereas beebread leads to worker bee fate. However， these determinants are not fully characterized. Here we report that plant RNAs， particularly miRNAs， which are more enriched in beebread than in royal jelly， delay development and decrease body and ovary size in honeybees， thereby preventing larval differentiation into queens and inducing development into worker bees. Mechanistic studies reveal that amTOR， a stimulatory gene in caste differentiation， is the direct target of miR162a. Interestingly， the same effect also exists in non-so... More
