Pulmonary immunization with a recombinant influenza A virus vaccine induces lung-resident CD4+ memory T cells that are associated with protection against tuberculosis.
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| Peptide Synthesis |
Cells were cultured at a density of less than 2 × 106cells/ml ofRPMI/10%FCS in an ELISpot plate pre-coated with 15 μg/ml ofanti-mouse IFNγmonoclonal antibody (clone AN18) in thepresence of pathogen-specific peptides, NP366–374andAg85B240–254(Genscript), at afinal concentration of 10 μg/ml orwith BCG lysate at afinal concentration of 5 μg/ml. |
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