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Reconstructed ancestral enzymes suggest long-term cooling of Earth's photic zone since the Archean

Frontiers in Microbiology,. 2017; 
Guo, Z., Huang, J., Yan, G., Lei, L., Wang, S., Yu, L., … Yang, J.
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Recombinant Proteins coli BL21 cells. The ORF42 protein was expressed under 0.1 mM isopropyl β-D1-thiogalactopyranoside (IPTG) induction at 16◦C overnight, purified from the soluble fraction using a Ni-nitrilotriacetic acid (NTA) column (Genscript, Nanjing, China) according to the manufacturer’s instructions, and then analyzed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (Gu et al., 2014) Get A Quote

摘要

Identification and Characterization of Dpo42, a Novel Depolymerase Derived from the Escherichia coli Phage vB_EcoM_ECOO78.

关键词

: bacteriophage, depolymerase, Escherichia coli, biofilm, beta-lactamase
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