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至今，GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次，处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果，再次证明GenScript 有能力帮助业内科学家Make research easy.

ThiN as a versatile domain of transcriptional repressors and catalytic enzymes of thiamine biosynthesis

Scientific Reports,. 2017; 
antigens Ziwei Xiao, Zhiyong Ye, Vikeramjeet Singh Tadwal, Meixin Shen & Ee Chee Ren
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Peptide Synthesis PBMCs were isolated with Ficoll-Paque PLUS (GE Healthcare) and maintained in RPMI 1640 supplemented with 5% human AB serum (Sigma), 100 IU/ ml penicillin-streptomycin (Gibco), 2 mM L-glutamine (Sigma) and 10mM HEPES (Sigma). For 14-day cultures, PBMCs (1×106 cells/ml) were incubated with 10µM peptides (GenScript) and supplemented with 25 U/ml rIL2 (R&D Systems) Briefly, recombinant human HLA class I allele (exon 2–4) and human β2 m were expressed and solubilized in 8 M urea. Stock peptides (Genscript, USA) at 10mg/ml DMSO were added to HLA and β2m in refolding buffer for 72h, followed by dialysis against 10mM Tris-HCl (pH 8.0) at 4°C overnight Get A Quote

摘要

Host CD8 T cell response to viral infections involves recognition of 8–10-mer peptides presented by MHC-I molecules. However, proteasomes generate predominantly 2–7-mer peptides, but the role of these peptides is largely unknown. Here, we show that single short peptides of

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