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Computationally optimized deimmunization libraries yield highly mutated enzymes with low immunogenicity and enhanced activity

PNAS Early Edition. 2017; 
Regina S. Salvata , Deeptak Vermab , Andrew S. Parkerb , Jack R. Kirscha , Seth A. Brooksa,c, Chris Bailey-Kelloggb,d,e,, and Karl E. Griswolda,d,e,f,
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Peptide Synthesis Degenerate codon libraries were synthesized by GenScript.. Peptides were ordered from GenScript and were greater than 85% pure. Biotinylated tracer peptides were purchased from 21st Century BiochemicalsLib10 and Lib30 were constructed by GenScript and delivered as pooled plasmid in a pET26b backbone Get A Quote

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