An essential role of high-molecular-weight kininogen in endotoxemia
| Products/Services Used |
Details |
Operation |
| Peptide Synthesis |
Rabbit polyclonal anti-HK D5
was generated by GenScript. The following
peptides were commercially synthesized by GenScript: VSP
PHTSMAPAQDEERDSGKEQGHTRRHDW (peptide
1), RDSGKEQGHTRRHDWGHEKQRKHNLGHGHK
(peptide 2), GHEKQRKHNLGHGHKHERDQGHGH
QRGHGL (peptide 3), HERDQGHGHQRGHGLGHG
HEQQHGLGHGHK (peptide 4), GHGHEQQHGLGH
GHKFKLDDDLEHQGGHVL (peptide 5), FKLDDD
LEHQGGHVLDHGHKHKHGHGHGKH (peptide 6),
DHGHKHKHGHGHGKHKNKGKKNGKHNGWK
(peptide 7), and KNKGKKNGKHNGWKTEHLASSSED
STTPS (peptide 8). Peptides 1–7 were derived from the
D5 domain of HK, and peptide 8 was derived from the N
terminus of the D6 domain. The protein
purity, size, and identity were verified by Coomassie brilliant
blue staining and immunoblotting using a mouse anti-His
antibody (catalog no. A00186; GenScript) with the use of a
positive control Multiple-tag (catalog no. M0101; GenScript)
and protein markers (catalog no. MM0908; GenScript).The hybridoma producing mAb C11C1 was generated as
previously described (Song et al., 2004; Khan et al., 2010)
and purified by GenScript. An anti-DHG15 antibody was
produced by GenScript, using a peptide (DHGHKHKHG
HGHGKHC conjugated with KLH) HPLC purified to
>96% purity as immunogen. |
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