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Pathogenic events in a nonhuman primate model of oral poliovirus infection leading to paralytic poliomyelitis

Journal of Virology. 2017; 
Ling Shen# Crystal Y. Chen#, Dan Huang#, Richard Wang , Meihong Zhang , Lixia Qian , Yanfen Zhu , Alvin zhuoran Zhang , Enzhuo Yang , Arwa Qaqish , Konstantin Chumakov , Diana Kouiavskaia , Marco Vignuzzi , Neal Nathanson , Andrew J. Macadam , Raul Andino , Olen Kew , Junfa Xu , and Zheng W. Chen*
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Recombinant Proteins Tissue sections were incubated for 30 min 178 with normal mouse serum and washed twice, and then incubated for 30 min with protein block 179 serum free medium. Sections were then incubated for 1 hour with diluted fluorescence-labeled 180 antibodies [APC-conjugated mouse anti-3D mAb, produced/characterized by GenScript and 181 Chen Lab; PE-conjugated mouse anti-human CD14, BD, Cat# 555398, or FITC-conjugated anti182 human CD11C, Biolegend, Cat# 301603]. Sections were scanned with a Confocal Microscope 183 Zeiss LSM 710 using a 40x water objective lens, and images were acquired with the Zensoftware. Get A Quote

摘要

Despite a great deal of prior research, the early pathogenic events in natural oral poliovirus 32 infection remain poorly defined. To establish a model for study, we infected 39 macaques 33 by feeding single high doses of the virulent Mahoney strain of wild type 1 poliovirus. Doses ranging from107 –109 34 TCID50 consistently infected all animals, and most monkeys receiving 108 or 109 35 TCID50 developed paralysis. There was no apparent difference in the 36 susceptibility of the three macaque species (rhesus, cynomolgus, and bonnet) used. Virus 37 excretion in stool and nasopharynges was consistently observed, with occasional viremia, and 38 virus was isolated from tonsils, gut mucosa, and draining lymph nodes... More

关键词

oral poliovirus infection, poliomyelitis, animal model, macaques
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