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Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay.

J Nanobiotechnology. 2019;

ShengYamin，WangKun，LuQizhong，JiPinpin，LiuBaoyuan，ZhuJiahong，LiuQingyuan，SunYani，ZhangJingfei，ZhouEn-Min，Zha

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Recombinant Proteins	… purified camel IgG [18], and the HRP-conjugated goat anti-mouse IgG was used as the third antibody (Jackson ImmunoResearch Laboratories, USA) … for their capacity to bind with the NDV-NP protein using an indirect ELISA with an anti-HA-Tag antibody (GenScript Biotech Corp …	Get A Quote

摘要

Sensitive and specific antibodies can be used as essential probes to develop competitive enzyme-linked immunosorbent assay (cELISA). However， traditional antibodies are difficult to produce， only available in limited quantities， and ineffective as enzymatic labels. Nanobodies， which are single-domain antibodies (sdAbs)， offer an alternative， more promising tool to circumvent these limitations. In the present work， a cELISA using nanobody-horseradish peroxidase (HRP) fusion protein firstly designed as a probe was developed for detecting anti-Newcastle disease virus (NDV) antibodies in chicken sera.