Cell phenotypes are closely related to the epigenome， which could be precisely regulated by the targeted manipulation of epigenetic marks. Here， we have successfully produced a targeted histone methylation system， which consists of nuclease-null dCas9 protein， the sgRNA fused with PP7 RNA aptamers and the Enhancer of Zeste Homolog 2 (EZH2) fused to PP7 coat protein (PCP). Guided by the dCas9/sgRNA-PP7， the PCP-EZH2 can specifically target gene loci to catalyze 3 methylation of histone H3 lysine 27， resulting in the inhibition of gene expression. This kind of gene inhibition system is supposed to be highly effective， specific and flexible. As a proof-of-concept study， sgRNA targeting C/ebpα promoter region was designed. In the cells co-infected with the dCas9， sgRNA/C/ebpα-PP7 and PCP-EZH2， the expression of C/ebpα gene was significantly reduced via induction of trimethylation to H3K27 on C/ebpα promoter， with the results epigenetically inherited in the daughter cells. In conclusion， our results successfully established a gene modification system consisting of dCas9/sgRNA-PP7 and PCP-EZH2， providing a robust tool for targeted manipulation of gene epigenetic modification and expression.