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Peptide Synthesis> | … 14. The peptides used in these experiments are synthetic peptides that were purchased at >95% purity from GenScript (San Diego, CA). 15 … 17. This can be achieved by either completing a separate control experiment or by using the average heat from several injections. 18 … | Get A Quote |
The three human plastins (L-plastin, T-plastin, and I-plastin) are important regulatory Ca-binding proteins that belong to the family of actin-binding proteins. Plastins are involved in the regulation of the actin cytoskeleton as well as the cross-linking of actin filaments. In addition to four calponin-homology (CH) domains, all three plastins contain two N-terminal EF-hand Ca-binding motifs which together are homologous to a single lobe of the well-known calcium-regulatory protein calmodulin. This part of the protein allows for the regulation of the actin bundling activity in response to elevated calcium levels. In this protocol, we describe the purification of the EF-hand headpiece domains of all thr... More