Products/Services Used | Details | Operation |
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Peptide Synthesis> | … Peptide Synthesis The synthetic pentapeptide Phr LS20 (NH 2 -QKGMY-COOH) was purchased from GenScript According to the recommendations of the manufacturer, the peptide was resuspended in H 2 O and stored at −20 °C Chemical Cross-linking of Rco … | Get A Quote |
Conjugation of plasmid pLS20 from Bacillus subtilis is limited to a time window between early and late exponential growth. Genetic evidence has suggested that pLS20-encoded protein RcoLS20 represses expression of a large conjugation operon, whereas Rap protein RapLS20 relieves repression. We show that RapLS20 is a true antirepressor protein that forms dimers in vivo and in vitro and that it directly binds to the repressor protein RcoLS20 in a 1:1 stoichiometry. We provide evidence that RapLS20 binds to the helix-turn-helix-containing domain of RcoLS20 in vivo, probably obstructing DNA binding of RcoLS20, as seen in competitive DNA binding experiments. The activity of RapLS20 in turn is counteracted by the... More