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Induction of Plasmid Conjugation in Bacillus subtilis Is Bistable and Driven by a Direct Interaction of a Rap/Phr Quorum-sensing System with a Master Repressor.

J. Biol. Chem.. 2015; 
RöschThomas C,GraumannPet
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Peptide Synthesis … Peptide Synthesis The synthetic pentapeptide Phr LS20 (NH 2 -QKGMY-COOH) was purchased from GenScript According to the recommendations of the manufacturer, the peptide was resuspended in H 2 O and stored at −20 °C Chemical Cross-linking of Rco … Get A Quote

摘要

Conjugation of plasmid pLS20 from Bacillus subtilis is limited to a time window between early and late exponential growth. Genetic evidence has suggested that pLS20-encoded protein RcoLS20 represses expression of a large conjugation operon, whereas Rap protein RapLS20 relieves repression. We show that RapLS20 is a true antirepressor protein that forms dimers in vivo and in vitro and that it directly binds to the repressor protein RcoLS20 in a 1:1 stoichiometry. We provide evidence that RapLS20 binds to the helix-turn-helix-containing domain of RcoLS20 in vivo, probably obstructing DNA binding of RcoLS20, as seen in competitive DNA binding experiments. The activity of RapLS20 in turn is counteracted by the... More

关键词

Bacillus subtilis,Rap-Phr module,bacterial conjugation,gene regulation,plasmid,protein-nucleic acid interaction,protein-protein interaction,quorum sen
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