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Stabilization of fungi-derived recombinant FAD-dependent glucose dehydrogenase by introducing a disulfide bond.

Biotechnol. Lett.. 2015-05; 
SakaiGenki，KojimaKatsuhiro，MoriKazushige，OonishiYosuke，Sode
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Codon Optimization … Materials and methods Gene and host The A flavus-derived FAD-GDH structural gene (NCBI reference sequence: XP_0023725991) was synthesized by GenScript Inc (Piscataway, NJ, USA), with codons optimized for expression in E coli … Get A Quote

摘要

To improve the stability of E. coli-produced non-glycosylated fungal FAD-glucose dehydrogenase induced a disulfide bond by site-directed mutagenesis based on structural comparisons with glucose oxidases.

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