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A fast and easy strategy for protein purification using "teabags".

Sci Rep. 2016; 
CastaldoM,BarlindL,MauritzsonF,WanP T,Snijde
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Proteins, Expression, Isolation and Analysis … Materials and Methods. Electrophoresis. Samples were denatured in LDS buffer, separated on 4–12% Bis-Tris NuPage gels (Thermo Scientific) and either stained with e-Stain device from GenScript or Instant Blue (Expedeon Ltd.). Choice of mesh … Get A Quote

摘要

Protein purification often involves affinity capture of proteins on stationary resin, alternatively proteins are captured on free flowing resin for subsequent separation from bulk fluid. Both methods require labour and time intensive separation of particulate matter from fluid. We present a method where affinity resin is contained within porous-walled containers, supporting clarification, product recovery, and concentration in a single step with minimal hands-on processing time, without significant investments in equipment.

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