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Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein.

PLoS ONE. 2009; 
VolkmannGerrit，LiuXiang
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Proteins, Expression, Isolation and Analysis After washing away unbound proteins with oSB, the resin was incubated in oSB containing 0.1 mM TCEP and either 45 µM IC-L (for MINC protein) or 125 µM IC-B (for EINC protein, sequence: GVFVHNSAGSK-biotin, purchased from GenScript), and the incubation was continued overnight at room temperature. Get A Quote

摘要

Site-specific protein labeling or modification can facilitate the characterization of proteins with respect to their structure， folding， and interaction with other proteins. However， current methods of site-specific protein labeling are few and with limitations， therefore new methods are needed to satisfy the increasing need and sophistications of protein labeling.

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