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Cascade synthesis of uridine-5'-diphosphate glucuronic acid by coupling multiple whole cells expressing hyperthermophilic enzymes.

Microb. Cell Fact.. 2019-07; 
MengDan-Hua，DuRan-Ran，ChenLu-Zhou，LiMeng-Ting，LiuFei，HouJin，ShiYi-Kang，WangFeng-Shan，ShengJu-Z
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Codon Optimization and TkNOX gene (GenBank accession number NC_006624.1) with a 5ʹ-His tag fragments were synthesized with codon optimization for gene expression in E. coli by GenScript Inc. (Nanjing, China), respectively. Get A Quote

摘要

Enzymatic glycan synthesis has leapt forward in recent years and a number of glucuronosyltransferase (EC 2.4.1.17) have been identified and prepared， which provides a guide to an efficient approach to prepare glycans containing glucuronic acid (GlcA) residues. The uridine 5'-diphosphate (UDP) activated form， UDP-GlcA， is the monosaccharide donor for these glucuronidation reactions.

关键词

Biocatalysis，Hyperthermophilic enzyme，NAD+ regeneration，Nucleotide sugar，UDP-GlcA，Whole cell synth
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