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The high genetic similarity between rhinoviruses and enteroviruses remains as a pitfall for molecular diagnostic tools: A three-year overview.

Infect. Genet. Evol.. 2019-08;

AndrésCristina，Pi?anaMaria，VilaJorgina，EsperalbaJuliana，Trejo-ZahínosJesús，CodinaMaria Gema，MartínMaria Carmen，FuentesFrancisco，RubioSusana，PumarolaTomàs，AntónAnd

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Gene Synthesis	… The performance was evaluated using pUC57 plasmids with inserts of the targeted viral or bacterial sequences, synthesized by by GenScript Corp. (Piscataway, NJ). Table 1. Primers and probes used in multiplex real-time PCR run in five reaction mixtures, AE …	Get A Quote

摘要

Enteroviruses (EVs) and rhinoviruses (RVs) belong to the Enterovirus genus within the Picornaviridae family， and show genetic similarities. These viruses are related to mild diseases， but EVs infections can sometimes lead to more severe complications. Current diagnostic molecular techniques should discriminate between the four EV and the three RV species that infect humans. The aim was to revise the EV and RV PCR-confirmed specimens by sequencing for genetic characterisation.