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Maternal antibody induced by recombinant gp85 protein vaccine adjuvanted with CpG-ODN protects against ALV-J early infection in chickens.

Vaccine. 2013; 
DouWenwen,LiHongmei,ChengZiqiang,ZhaoPeng,LiuJianzhu,CuiZhizhong,LiuHaigang,JingWeifang,GuoHu
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Proteins, Expression, Isolation and Analysis … The soluble fraction was harvested and applied to a high-affinity Ni-NTA column (GenScript USA Inc., Nanjing, China). The eluted proteins were further purified by running the eluate through an SD200 gel filtration column (GenScript Get A Quote

摘要

In this study, the efficacy of a recombinant protein vaccine encoding the gp85 gene from the subgroup J avian leukosis virus (ALV-J) co-administered with cytosine-phosphate-guanine oligodeoxynucleotide (CpG-ODN) or Freund's adjuvants was investigated for the protection against early ALV-J infection in chickens. The gp85 gene from ALV-J was amplified using polymerase chain reaction (PCR), and the recombinant protein was expressed in Escherichia coli. The purified recombinant protein was injected intramuscularly into the breeder hens along with CpG-ODN or Freund's adjuvants, and the antibodies in the serum were assayed regularly post inoculation. The fertilized eggs from the vaccinated hens were hatched, ... More

关键词

CpG-ODN,Immunoprotection,Maternal antibody,Recombinant gp85 protein,Subgroup J avian leukosis virus (AL
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