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Development of a NS2B/NS3 protease inhibition assay using AlphaScreen beads for screening of anti-dengue activities.

Heliyon. 2018;

AbduramanMuhammad Asyraf，HarionoMaywan，YusofRohana，RahmanNoorsaadah Abd，WahabHabibah A，TanMei

Products/Services Used	Details	Operation
Peptide Synthesis	… Merck (Darmstadt, Germany). Peptide substrate (GGGFKEFAAGRKSLTLNLITEGGG) was synthesized and undergone quality control analysis using Mass Spectrometry and HPLC by GenScript (NJ, USA). 4-fluorobenzaldehyde …	Get A Quote

摘要

Dengue infection is an endemic infectious disease and it can lead to dengue fever， dengue hemorrhagic fever， and/or dengue shock syndromes. Dengue NS2B/NS3 protease complex is essential for viral replication and is a primary target for anti-dengue drug development. In this study， a NS2B/NS3 protease inhibition assay was developed using AlphaScreen beads and was used to screen compounds for their protease inhibition activities.