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Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate.

Microb. Cell Fact.. 2010; 
JacobsPieter P，InanMehmet，FestjensNele，HaustraeteJurgen，Van HeckeAnnelies，ContrerasRoland，MeagherMichael M，Callewaert
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Cellular Analysis … product peak by ultrafiltration (Vivaspin 20, 3000 MWCO; Sartorius Stedim Biotech), the mGM-CSF concentration was determined using the BCA assay (Pierce) and endotoxin levels were determined with the ToxinSensor Chromogenic LAL Endotoxin Assay Kit (GenScript USA) … Get A Quote

摘要

Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous， defined N-glycans. However， data on the behaviour of these strains in high cell density cultivation are scarce.

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