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Induced novel psbA mutation (Ala to Thr) in higher plants confers resistance to PSII inhibitor metribuzin in Lens culinaris.

Pest Manag Sci. 2019-06;

McMurrayLarn S，PrestonChristopher，VandenbergAlbert，MaoDili，BettKirstin E，PaullJeffr

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Nucleic Acid Purification & Analysis	… 0.4 μl of each primer, 0.4 μl dNTPs, 1.6 μM MgCl2, 1 unit of Genscript Taq Polymerase and 50 ng of template DNA … Polymerase chain reaction products were mixed with GenScript GelRed loading dye then run on a 1% agarose gel electrophoresis with Tris-acetate (TAE) buffer …	Get A Quote

摘要

Weed competition is a major limitation to worldwide lentil (Lens culinaris Medik.) production in part due to limited effective safe herbicide options. Metribuzin is a photosystem II inhibiting herbicide that provides broad spectrum weed control， however it causes excessive injury in lentil. Dose response analysis of photosystem II inhibiting herbicides and DNA sequencing of the psbA chloroplast gene occurred to quantify the spectrum and mechanism of herbicide resistance in two ethyl-methanesulfonate (EMS) induced mutant lentils.