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Role of calcium in regulating the intra- and extracellular cleavage of von Willebrand factor by the protease ADAMTS13.

Blood Adv. 2017; 
Gogia Shobhit,Kelkar Anju,Zhang Changjie,Dayananda Kannayakanahalli M,Neelamegham Sr
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Catalog Antibody (Genscript), or (3) anti-tetra His mAb (Qiagen). Membranes were then incubated with appropriate Get A Quote

摘要

von Willebrand factor (VWF) and the metalloprotease a disintegrin and metalloprotease with thrombospondin type 1 motif 13 (ADAMTS13) are present both within endothelial cells (ECs) and in peripheral blood. Calcium concentrations are lower in intracellular compartments (80-400 μM) compared with the extracellular milieu (~1.25 mM). Because low calcium favors VWF A2-domain proteolysis by ADAMTS13, the dependence of proteolysis rates on calcium was assayed both within ECs and in blood. Confocal microscopy studies demonstrate partial perinuclear colocalization of VWF with ADAMTS13 in human umbilical vein ECs (HUVECs). Consequently, low levels (5%-10%) of VWF cleavage products were detected in HUVEC lysates an... More

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