| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | TCR-transfected Jurkat effector cells (50,000 cells) were co-cultured with 50,000 target cells either pulsed with 20 µM TL9 peptide (purchased from GenScript at >90% purity) or infected with HIV-1 in a total volume of 100 µl, and TCR recognition activity was quan- tified by luminescence after 6 h (Tecan M200).... To screen antigen cross-recognition, a peptide panel consisting of all single amino acid TL9 variants (180 total peptides) was purchased from GenScript. | Get A Quote |
Some closely related human leukocyte antigen (HLA) alleles are associated with variable clinical outcomes following HIV-1 infection despite presenting the same viral epitopes. Mechanisms underlying these differences remain unclear but may be due to intrinsic characteristics of the HLA alleles or responding T cell repertoires. Here we examine CD8 T cell responses against the immunodominant HIV-1 Gag epitope TL9 (TPQDLNTML) in the context of the protective allele B*81:01 and the less protective allele B*42:01. We observe a population of dual-reactive T cells that recognize TL9 presented by both B*81:01 and B*42:01 in individuals lacking one allele. The presence of dual-reactive T cells is associated with lower pl... More
