| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | NK Cell Cytotoxicity Assay Using T2 Cell Lines To test the effect of specific peptides, T2 cells were incubated in serum-free RPMI media overnight at 26 C in the presence of 100 mM synthetic peptide (GenScript).... T2 cells (3 3 104) were incubated with 100 mM peptide (Genscript) overnight in serum-free media at 26 C, washed, and re-suspended in serum-free media with anti-CD107a-TexasRed antibody (eBioscience). | Get A Quote |
HLA-B46:01 was formed by an intergenic mini-conversion， between HLA-B15:01 and HLA-C01:02， in Southeast Asia during the last 50，000 years， and it has since become the most common HLA-B allele in the region. A functional effect of the mini-conversion was introduction of the C1 epitope into HLA-B46:01， making it an exceptional HLA-B allotype that is recognized by the C1-specific natural killer (NK) cell receptor KIR2DL3. High-resolution mass spectrometry showed that HLA-B46:01 has a low-diversity peptidome that is distinct from those of its parents. A minority (21%) of HLA-B46:01 peptides， with common C-terminal characteristics， form ligands for KIR2DL3. The HLA-B46:01 peptidome is predicted to be e... More
