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Intramolecular platinum migration on a peptide in gas phase during collision-induced dissociation.

J Inorg Biochem. 2020;

Du Z1, Qu Y2, Farrell NP3.

Products/Services Used	Details	Operation
Proteins, Expression, Isolation and Analysis	… Laboratory, USA). All reagents were purchased from Sigma Aldrich, USA and used without further purification. The peptide (ACPECP) was purchased from GenScript Corporation. 2.2. Mass spectrometry. For mass spectrometry …	Get A Quote

摘要

We report the migration of platinum ligand unit {Pt(en)}2 (en = ethylenediamine) on a short peptide during collision-induced dissociation fragmentation combined with the characterization of the same species by 2D [1H,15N] HSQC (Heteronuclear Single Quantum Coherence) NMR spectroscopy. The NMR spectrum showed that the cysteine is platinated while the MS/MS (Tandem mass spectrometry) showed the platination at glutamic acid. Our results provide the first experimental evidence of platinum migration on peptide during collision-induced dissociation.,Copyright © 2019 Elsevier Inc. All rights reserved.