Galaxy银河|澳门官网·登录入口

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

An engineered variant of SETD3 methyltransferase alters target specificity from histidine to lysine methylation

J Biol Chem. 2020; 
Dai S, Horton JR, Wilkinson AW, Gozani O, Zhang X, Cheng X.
Products/Services Used Details Operation
Peptide Synthesis … Page 7 7 (pH 80), 200 mM NaCl, 5% glycerol, and 05 mM tris (2-carboxyethyl) phosphine (TCEP) and kept at −80°C for future use The actin peptides (residues 66–80 or 66-88) containing H73, K73 or Q73 were purchased from Genscript Crystallography … Get A Quote

摘要

Most characterized SET domain (SETD) proteins are protein lysine methyltransferases, but SETD3 was recently demonstrated to be a protein (i.e.actin) histidine-N3methyltransferase. Human SETD3 shares a high structural homology with two known protein lysine methyltransferases-human SETD6 and the plant LSMT-but differs in the residues constituting the active site. In the SETD3 active site, Asn-255 engages in a unique hydrogen-bonding interaction with the target histidine of actin that likely contributes to its >1300-fold greater catalytic efficiency (kcat/Km) on histidine than on lysine. Here, we engineered active-site variants to switch the SETD3 target specificity from histidine to lysine.  Substitution of Asn-... More

关键词

S-adenosylmethionine (SAM); enzyme catalysis; enzyme kinetics; glutamine methylation; histidine; histidine methylation; lysine methylation; protein methylation; structural biology
XML 地图