Products/Services Used | Details | Operation |
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Catalog Antibody> | Primary antibodies; anti-GAPDH (Santa Cruz Biotechnology), anti-HK1, anti-HK2, and anti-Bax (Abcam), anti-Bcl-2 (Sigma–Aldrich), anti-Akt, anti-phospho-Akt (Ser473), and anti-phospho-Akt (Thr308) (GenScript, Antibodies-online GmbH), anti-pAMPKα (Thr172) (cell signaling) were visu- alized by following secondary antibody; anti-rabbit (GE Healthcare Amersham), anti-mouse (Thermo Fisher Scien- tific), or anti-goat (Santa Cruz Biotechnology) and Super- Signal West Dura Extended Duration Substrate (Thermo Fisher Scientific) using LAS-4000 imaging system (Genet- ica, Fujifilm). | Get A Quote |
Adaptation to chronic hypoxia represents a potential cardioprotective intervention reducing the extent of acute ischemia/reperfusion (I/R) injury, which is a major cause of death worldwide. The main objective of this study was to investigate the anti-apoptotic Akt/hexokinase 2 (HK2) pathway in hypoxic hearts subjected to I/R insult. Hearts isolated from male Wistar rats exposed either to continuous normobaric hypoxia (CNH; 10% O2) or to room air for 3 weeks were perfused according to Langendorff and subjected to 10 min of no-flow ischemia and 10 min of reperfusion. The hearts were collected either after ischemia or after reperfusion and used for protein analyses and quantitative fluorescence microscopy. The ... More