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Adaptation to chronic continuous hypoxia potentiates Akt/HK2 anti-apoptotic pathway during brief myocardial ischemia/reperfusion insult.

Mol Cell Biochem. 2017; 
Kolar D, Gresikova M, Waskova-Arnostova P, Elsnicova B, Kohutova J, Hornikova D, Vebr P, Neckar J, Blahova T, Kasparova D, Novotny J, Kolar F, Novakova O, Zurmanova JM.
Products/Services Used Details Operation
Catalog Antibody Primary antibodies; anti-GAPDH (Santa Cruz Biotechnology), anti-HK1, anti-HK2, and anti-Bax (Abcam), anti-Bcl-2 (Sigma–Aldrich), anti-Akt, anti-phospho-Akt (Ser473), and anti-phospho-Akt (Thr308) (GenScript, Antibodies-online GmbH), anti-pAMPKα (Thr172) (cell signaling) were visu- alized by following secondary antibody; anti-rabbit (GE Healthcare Amersham), anti-mouse (Thermo Fisher Scien- tific), or anti-goat (Santa Cruz Biotechnology) and Super- Signal West Dura Extended Duration Substrate (Thermo Fisher Scientific) using LAS-4000 imaging system (Genet- ica, Fujifilm). Get A Quote

摘要

Adaptation to chronic hypoxia represents a potential cardioprotective intervention reducing the extent of acute ischemia/reperfusion (I/R) injury, which is a major cause of death worldwide. The main objective of this study was to investigate the anti-apoptotic Akt/hexokinase 2 (HK2) pathway in hypoxic hearts subjected to I/R insult. Hearts isolated from male Wistar rats exposed either to continuous normobaric hypoxia (CNH; 10% O2) or to room air for 3 weeks were perfused according to Langendorff and subjected to 10 min of no-flow ischemia and 10 min of reperfusion. The hearts were collected either after ischemia or after reperfusion and used for protein analyses and quantitative fluorescence microscopy. The ... More

关键词

Heart; Hexokinase; Hypoxia; Ischemia/reperfusion; Mitochondria; Protein kinase B/Akt
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