Galaxy银河|澳门官网·登录入口

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A high yielding IFNAR1 ECD mammalian expression process for use in autoimmune disease drug development.

Protein Expr Purif. 2020; 
Kittinger C, Barnes A, Hunter A, Machiesky L, Phipps S, Shannon A, Stadelman R, Wilson S, O'Connor E.
Products/Services Used Details Operation
Catalog Antibody … MA, USA). The primary antibody used was His Tag [Biotin], pAb, rabbit (GenScript; Piscataway, NJ, USA) and the secondary reagent was Peroxidase labeled Streptavidin (Thermo Fisher Scientific; Waltham, MA, USA). The membrane … Get A Quote

摘要

Interferon-alpha receptor 1 (IFNAR1) is a target of interest for recombinant biotherapeutics that block the JAK/STAT pathway. This pathway is believed to play a role in many diseases including Hepatitis B and C, Herpes Simplex, Multiple Sclerosis, and other autoimmune disorders. By using IFNAR1 as a target to block Type I IFN from binding to the JAK/STAT pathway and prevent activation of this target, autoimmune disease progression can be modulated. Current IFNAR1 extracellular domain (ECD) expression and purification protocols are labor intensive with low product yield and limited scalability. In this work, we evaluate three different expression systems (baculovirus, human embryonic kidney 293 (HEK293×), and C... More

关键词

CHO expression; IFNAR1 ECD; IMAC; Immobilized metal affinity chromatography; Interferon-alpha receptor 1 extracellular domain; Mixed mode chromatography
XML 地图