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Recombinant Proteins> | … were then transferred onto a nitrocellulose membrane, blocked with TBST [TBS, 005% (v/v) Tween-20] containing 5% (w/v) skim milk, and incubated with rabbit anti-IcsA antibody [28], rabbit anti-GST antibody (in house made), or mouse anti-His antibody (Genscript) for 4 h The … | Get A Quote |
Shigella species cause bacillary dysentery, especially among young individuals. Shigellae target the human colon for invasion; however, the initial adhesion mechanism is poorly understood. The Shigella surface protein IcsA, in addition to its role in actin-based motility, acts as a host cell adhesin through unknown mechanism(s). Here we confirmed the role of IcsA in cell adhesion and defined the region required for IcsA adhesin activity. Purified IcsA passenger domain was able block S. flexneri adherence and was also used as a molecular probe that recognised multiple components from host cells. The region within IcsA's functional passenger domain (aa 138-148) was identified by mutagenesis. Upon the deletion of ... More