| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | The pristine GO was synthesized by modified Hummer’s method. 40 mg of the obtained GO was suspended in 40 mL dimethylformamide, and then mixed with 3.5 mg of (3-hydroxy-3H-1,2,3-triazolo[4,5-b]pyridinato-O)tri-1-pyrrolidinylphosphonium hexafluorophosphate (PyAOP), 10 mg N,N-diisopropylethylamine, and 5 mg of the antimicrobial peptide D28 (FLGVVFKLASKVFPAVFGKV, Genscript, China). The suspension was sonicated for 10 min, and stirred for 12 h at 20 °C. The reaction liquid was then centrifuged at 12, 000 rpm for 20 min. The pellets were washed with DMF and ethanol, and dried at the room temperature, obtaining the antimicrobial peptide-grafted GO (D28@GO) for further experiments. | Get A Quote |
Graphene nanomaterials have rapidly developed in recent years. However, its antimicrobial activity does not achieve satisfactory effect. During the past years, it has been found that antimicrobial peptides (AMP) have a good inhibitory effect on pathogenic microbes. In this study, we synthesized AMP-grafted graphene oxide (GO) nanosheets, i.e., D28@GO, against the pathogens such as Candida albicans and Escherichia coli. Growth inhibition assays showed that D28@GO had strong inhibitory effect against the pathogens and this toxicity contributes to cell membrane damage caused by interaction between D28@GO and macrobiomolecules. In vivo experiments further showed that D28@GO severely suppressed systemic infecti... More
